Ordering Guidance

This test is appropriate for patients presenting with proximal muscle weakness, elevated muscle enzymes (eg, creatine kinase), and/or suggestive cutaneous features (eg, heliotrope rash, Gottron’s papules) consistent with myositis and related disorders. This test can assist in classifying IIM subtypes (eg, dermatomyositis, anti-synthetase syndrome, immune-mediated necrotizing myopathy, or inclusion body myositis. It may be useful in distinguishing idiopathic inflammatory myopathy from overlapping connective tissue diseases (CTDs). For patients with suspicion of overlap syndromes with CTDs additional myositis-associated antibody testing may be warranted beyond this panel.

Additional Testing Requirements

In patients with atypical or non-classical presentations testing for some myositis associated antibodies may be considered including anti-U1-snRNP, PM/Scl, Ro52 or Ro60.

Necessary Information

Provide the following information:

-Relevant clinical information

-Ordering provider name, phone number, mailing address, and e-mail address

Specimen Required

Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 2.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.