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Test ID BLOD0204 Pyridoxal 5-Phosphate, Plasma

Important Note

Note:  Fasting overnight (12-14 hours). Infants-collect prior to next feeding. Water can be taken as needed.  For 24 hours prior to specimen collection, patient must not take multivitamins or vitamin supplements.


Green top (Sodium or Lithium Heparin) Plasma, Protect from light and FROZEN


Collection Instructions:
1. Centrifuge at 4° C within 2 hours of collection.
2. Aliquot all plasma into amber vial and freeze immediately.
 

Secondary ID

42359

Useful For

Determining the concentration of pyridoxal 5-phosphate in the assessment of vitamin B6 status

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Pyridoxal 5-Phosphate (PLP), P

Specimen Type

Plasma Heparin


Shipping Instructions


Ship specimen in amber vial to protect from light.



Specimen Required


Patient Preparation:

1. Patient should fast overnight (12-14 hours); infants-should have specimen collected before next feeding. Water can be taken as needed.

2. For 24 hours before specimen collection, patient must not take multivitamins or vitamin supplements.

Supplies: Amber Frosted Tube, 5 mL (T915)

Collection Container/Tube: Green top (sodium or lithium heparin) or plasma gel separator (PST)

Submission Container/Tube: Amber vial

Specimen Volume: 1 mL

Collection Instructions:

1. Centrifuge at 4° C within 2 hours of collection.

2. Aliquot all plasma into amber vial and freeze immediately.


Specimen Minimum Volume

0.75 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma Heparin Frozen 29 days LIGHT PROTECTED

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Reference Values

5-50 mcg/L

Day(s) Performed

Monday through Thursday, Saturday, Sunday

Report Available

1 to 7 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

84207

Forms

If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.