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Test ID NBLD0506 Toxoplasma gondii, Molecular Detection, PCR, Varies

Reporting Name

Toxoplasma gondii PCR

Useful For

Supporting the diagnosis of acute cerebral, ocular, disseminated, or congenital toxoplasmosis

 

This test should not be used to screen healthy patients.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Varies


Necessary Information


Specimen source is required.



Specimen Required


Submit only 1 of the following specimens:

 

Specimen Type: Amniotic fluid

Container/Tube: Sterile container

Specimen Volume: 0.5 mL

Collection Instructions: Do not centrifuge.

 

Specimen Type: Spinal fluid

Supplies: Sarstedt Aliquot Tube 5 mL (T914)

Container/Tube:

Preferred: 12 x 75-mm screw cap vial

Acceptable: Sterile vial

Specimen Volume: 0.5 mL

Collection Instructions: Do not centrifuge.

 

Specimen Type: Fresh tissue

Supplies:M4-RT (T605)

Container/Tube:

Preferred: Multi-microbe medium (eg, M4-RT)

Acceptable: Sterile container with 1 to 2 mL of sterile saline

Specimen Volume: Entire collection

Collection Instructions: Submit only fresh tissue in a sterile container containing 1 mL to 2 mL of sterile saline or multi-microbe medium (M4-RT, M4, or M5)

 

Specimen Type: Ocular fluid

Supplies: Sarstedt Aliquot Tube 5 mL (T914)

Collection Container: 12 x 75-mm screw cap vial

Specimen Volume: 0.3 mL

Collection Instructions:

1. Aliquot collected fluid into screw-cap vial. Do not submit ocular fluid in syringe.

2. Do not centrifuge or dilute the specimen.


Specimen Minimum Volume

Amniotic Fluid, Ocular Fluid, Spinal Fluid: 0.3 mL
Tissue: 2 × 2 mm biopsy

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reference Values

Negative

Day(s) Performed

Monday through Saturday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87798

Report Available

Same day/1 to 4 days

Reject Due To

Heat-inactivated specimen Reject

Method Name

Polymerase Chain Reaction (PCR)/DNA Probe Hybridization

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Testing Algorithm

For more information see Meningitis/Encephalitis Panel Algorithm

Secondary ID

81795