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Test ID BLOD0360 Testosterone, Total, Bioavailable, and Free, Serum

Reporting Name

Testosterone, Total, Bio, Free, S

Useful For

Second- or third-order test for evaluating testosterone status (eg, when abnormalities of sex hormone-binding globulin are present)

Profile Information

Test ID Reporting Name Available Separately Always Performed
TTST Testosterone, Total, S Yes Yes
FRTST Testosterone, Free, S No Yes
BATS Testosterone, Bioavailable, S No Yes

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Serum


Specimen Required


Container/Tube: 

Preferred: Red top

Acceptable: Serum gel

Specimen Volume: 3.5 mL

Additional Information: Patient's age and sex are required.


Specimen Minimum Volume

2 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 14 days
  Frozen  60 days

Reference Values

TESTOSTERONE, TOTAL

Males        

0-5 months: 75-400 ng/dL

6 months-9 years: <7-20 ng/dL

10-11 years: <7-130 ng/dL

12-13 years: <7-800 ng/dL

14 years: <7-1,200 ng/dL

15-16 years: 100-1,200 ng/dL

17-18 years: 300-1,200 ng/dL

≥19 years: 240-950 ng/dL      

Tanner Stages*                            

I (prepubertal): <7-20

II: 8-66

III: 26-800

IV: 85-1,200      

V (young adult): 300-950

 

Females           

0-5 months: 20-80 ng/dL

6 months-9 years: <7-20 ng/dL

10-11 years: <7-44 ng/dL

12-16 years: <7-75 ng/dL

17-18 years: 20-75 ng/dL

≥19 years: 8-60 ng/dL

Tanner Stages*

I (prepubertal): <7-20

II: <7-47

III: 17-75

IV: 20-75

V (young adult): 12-60

 

*Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for boys at a median age of 11.5 (±2) years and for girls at a median age of 10.5 (±2) years. There is evidence that it may occur up to 1 year earlier in obese girls and in African American girls. For boys, there is no definite proven relationship between puberty onset and body weight or ethnic origin. Progression through Tanner stages is variable. Tanner stage V (young adult) should be reached by age 18.

 

TESTOSTERONE, FREE

Males (adult):

20 - <25 years: 5.25-20.7 ng/dL

25 - <30 years: 5.05-19.8 ng/dL

30 - <35 years: 4.85-19.0 ng/dL

35 - <40 years: 4.65-18.1 ng/dL

40 - <45 years: 4.46-17.1 ng/dL

45 - <50 years: 4.26-16.4 ng/dL

50 - <55 years: 4.06-15.6 ng/dL

55 - <60 years: 3.87-14.7 ng/dL

60 - <65 years: 3.67-13.9 ng/dL

65 - <70 years: 3.47-13.0 ng/dL

70 - <75 years: 3.28-12.2 ng/dL

75 - <80 years: 3.08-11.3 ng/dL

80 - <85 years: 2.88-10.5 ng/dL

85 - <90 years: 2.69-9.61 ng/dL

90 - <95 years: 2.49-8.76 ng/dL

95-100+ years: 2.29-7.91 ng/dL

 

Males (children):

<1 year: Term infants

 

1 to 15 days: 0.20-3.10 ng/dL*

16 days to 1 year: Values decrease gradually from newborn (0.20-3.10 ng/dL) to prepubertal levels

*Citation: J Clin Endocrinol Metab 1973;36(6):1132-1142

 

1-8 years: <0.04-0.11 ng/dL

9 years: <0.04-0.45 ng/dL

10 years: <0.04-1.26 ng/dL

11 years: <0.04-5.52 ng/dL

12 years: <0.04-9.28 ng/dL

13 years: <0.04-12.6 ng/dL

14 years: 0.48-15.3 ng/dL

15 years: 1.62-17.7 ng/dL

16 years: 2.93-19.5 ng/dL

17 years: 4.28-20.9 ng/dL

18 years: 5.40-21.8 ng/dL

19 years: 5.36-21.2 ng/dL

 

Females (adult):

20 - <25 years: 0.06-1.08 ng/dL

25 - <30 years: 0.06-1.06 ng/dL

30 - <35 years: 0.06-1.03 ng/dL

35 - <40 years: 0.06-1.00 ng/dL

40 - <45 years: 0.06-0.98 ng/dL

45 - <50 years: 0.06-0.95 ng/dL

50 - <55 years: 0.06-0.92 ng/dL

55 - <60 years: 0.06-0.90 ng/dL

60 - <65 years: 0.06-0.87 ng/dL

65 - <70 years: 0.06-0.84 ng/dL

70 - <75 years: 0.06-0.82 ng/dL

75 - <80 years: 0.06-0.79 ng/dL

80 - <85 years: 0.06-0.76 ng/dL

85 - <90 years: 0.06-0.73 ng/dL

90 - <95 years: 0.06-0.71 ng/dL

95-100+ years: 0.06-0.68 ng/dL

 

Females (children):

<1 year: Term infants

 

1 to 15 days: 0.06-0.25 ng/dL*

16 days to 1 year: Values decrease gradually from newborn (0.06-0.25 ng/dL) to prepubertal levels

*Citation: J Clin Endocrinol Metab 1973;36(6):1132-1142

 

1-4 years: <0.04 ng/dL

5 years: <0.04-0.07 ng/dL

6 years: <0.04-0.14 ng/dL

7 years: <0.04-0.23 ng/dL

8 years: <0.04-0.34 ng/dL

9 years: <0.04-0.46 ng/dL

10 years: <0.04-0.59 ng/dL

11 years: <0.04-0.72 ng/dL

12 years: <0.04-0.84 ng/dL

13 years: <0.04-0.96 ng/dL

14 years: <0.04-1.06 ng/dL

15-18 years: <0.04-1.09 ng/dL

19 years: 0.06-1.08 ng/dL

 

TESTOSTERONE, BIOAVAILABLE

Males

≤19 years: not established

20-29 years: 83-257 ng/dL

30-39 years: 72-235 ng/dL

40-49 years: 61-213 ng/dL

50-59 years: 50-190 ng/dL

60-69 years: 40-168 ng/dL

≥70 years: not established

Females (non-oophorectomized)

≤19 years: not established

20-50 years (on oral estrogen): 0.8-4.0 ng/dL

20-50 years (not on oral estrogen): 0.8-10 ng/dL

>50 years: not established

Day(s) and Time(s) Performed

Testosterone, bioavailable: Monday through Friday

Testosterone, free: Monday through Saturday, 1 p.m.

Testosterone, total: Monday through Saturday, 1 p.m.

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

84402-Testosterone, Free

84403-Testosterone, Total

84410-Testosterone, Bioavailable

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TTFB Testosterone, Total, Bio, Free, S 58716-2

 

Result ID Test Result Name Result LOINC Value
82978 Testosterone, Bioavailable, S 2990-0
3631 Testosterone, Free, S 2991-8
8533 Testosterone, Total, S 2986-8

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

Mild reject; Gross reject

Icterus

Mild OK; Gross reject

Other

NA

Method Name

FRTST: Equilibrium Dialysis

TTST: Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

BATS: Differential Precipitation